


Greenhouse gases (GHGs) are gases in the atmosphere that trap heat from the sun. Without these gases, the Earth’s surface would be much colder. GHGs are typically measured in carbon dioxide equivalents (CO₂e), which provides a way to express their impact on climate change based on their relative ability to trap heat.
Methane (CH₄) is a potent greenhouse gas (GHG) produced mainly during digestion in ruminant animals (i.e., enteric fermentation) such as cows, sheep, and goats. Over a 100-year period, methane is about 28 times more powerful than CO₂ at trapping heat in the atmosphere, making it a major contributor to climate change.
Enteric fermentation is a natural digestive process that occurs in the stomachs of ruminant animals, such as cows, sheep, and goats. These animals have specialized stomachs with multiple compartments, including the rumen. During digestion, methane (CH₄) is produced as a by-product and released primarily through burping.
In vitro testing is conducted outside living animals, in a controlled laboratory environment. For AnaCardex, this means testing the product in conditions that simulate the cow’s stomach (i.e., the rumen) using rumen fluids in bioreactors. The in vitro tests have shown highly promising results, reducing methane emissions by 67–97%.
In vivo testing is conducted inside living animals, in a real production environment. These tests confirm whether AnaCardex® performs effectively in practice. After the success of in vitro tests (i.e., tests conducted outside living animals, in a laboratory environment), the product is soon ready to progress to in vivo testing.
TRL stands for technology readiness level. It is a scale ranging from 1 to 9 used to assess the maturity of a new product. AnaCardex is estimated at TRL5, meaning the product has been tested in vitro (i.e., simulated rumen conditions) and is soon ready to progress to TRL 6 for in vivo testing (i.e., real production conditions).







AnaCardex® has the potential to reduce methane emissions by at least 50%, outperforming all competing product, which typically achieve reductions of 30% or less. This superior performance is driven by an innovative nano-carrier delivery system with targeted release.
AnaCardex® uniquely combines low production costs with carbon credit financing, making it notably more affordable than competing solutions. By using cashew by-products and offsetting costs through carbon credits, it is accessible and financially viable for farmers.
AnaCardex® is based on bioactive compounds, making it suitable for both organic and conventional farming systems. On the contrary, the current market leader is synthetic, and on-farm trials indicate that it is even potentially harmful, raising concerns for the health of the cattle.


Klaus
Kroll
Steen B.
Iversen
Jimmy
Sølvsten
J. Katerine Rodriguez
Mikael T. Christensen
Hans-Henrik Pedersen
Eleftherios Katsamakas
Natasha
Jørgensen
Freya Asp-Christensen
Mikkel
Sefron
Christina Chouchouli
Anine Bay Pedersen